LOCUS       40924_31630       12262 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB235 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 12262)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 12262)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12262)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        794..1139
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1156..1869
                     /codon_start=1
                     /label=TagRFP-T
                     /note="photostable variant of TagRFP (Shaner et al., 2008)"
                     /translation="ELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALKLV
                     GGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYCDL
                     PSKLGHKLELSTSLYK"
     protein_bind    1855..1979
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2016..2046
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2100..2756
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3101..3403
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3447..3571)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3603..3855
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3889..3905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3913..3929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3937..3967)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3982..4003)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4088..4260)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4320..4850)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4913..5589)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(5784..5808)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6329..7117
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7366..7954
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8140..8280)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8624..8818)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8887..9957)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10389..11015)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttagat tagccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg
      361 cttacgcagc aggtctcatc aagacgatct acccgagcaa taatctccag gaaatcaaat
      421 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
      481 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
      541 tgcttcacaa accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg
      601 aatcaaaggc catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga
      661 ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca
      721 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      781 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      841 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      901 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
      961 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     1021 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     1081 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac
     1141 gagcttacaa tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     1201 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     1261 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     1321 ctggctacca gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc
     1381 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     1441 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     1501 aacgtcaaga tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     1561 ctcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga
     1621 agcgacatgg ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca
     1681 tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac
     1741 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct
     1801 gtggccagat actgcgacct ccctagcaaa ctggggcaca agctagagtt atcaacaagt
     1861 ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag
     1921 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg
     1981 cggcccctcg aggggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     2041 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     2101 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2161 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     2221 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     2281 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     2341 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     2401 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     2461 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     2521 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     2581 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     2641 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     2701 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     2761 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     2821 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     2881 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     2941 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     3001 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     3061 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     3121 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     3181 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtccc
     3241 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     3301 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     3361 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     3421 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     3481 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     3541 tttctcgttc agctttcttg tacaaagtgg ttgataacag cgcttagagc tcgaatttcc
     3601 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     3661 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     3721 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     3781 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     3841 ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatggt catagctgtt
     3901 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3961 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc
     4021 ctatttctga attcaacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
     4081 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagatt ttattgatag
     4141 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     4201 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
     4261 ctcgagcttg tcgatcgact ctaggccggg aagccgatct cggcttgaac gaattgttag
     4321 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
     4381 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
     4441 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
     4501 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
     4561 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     4621 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     4681 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     4741 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     4801 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaccat ggtcgatcga
     4861 cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg
     4921 tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
     4981 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
     5041 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     5101 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
     5161 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
     5221 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     5281 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttca
     5341 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
     5401 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
     5461 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
     5521 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
     5581 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
     5641 agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa ccgaattcag
     5701 aaataaattc agcctaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta
     5761 ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa
     5821 cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc
     5881 cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg
     5941 ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg
     6001 tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaattc gggcacgaac
     6061 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     6121 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     6181 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     6241 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     6301 cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac
     6361 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     6421 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     6481 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     6541 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     6601 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     6661 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     6721 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     6781 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     6841 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     6901 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     6961 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     7021 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     7081 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt
     7141 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa
     7201 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata
     7261 agccctacac aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc
     7321 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7381 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7441 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7501 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7561 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7621 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7681 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7741 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7801 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7861 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7921 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7981 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8041 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8101 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     8161 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     8221 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     8281 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     8341 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     8401 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     8461 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     8521 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     8581 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     8641 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     8701 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     8761 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     8821 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     8881 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     8941 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     9001 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     9061 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     9121 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     9181 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     9241 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     9301 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     9361 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     9421 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     9481 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     9541 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     9601 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     9661 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     9721 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     9781 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     9841 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     9901 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     9961 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt
    10021 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
    10081 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
    10141 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
    10201 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
    10261 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
    10321 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
    10381 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
    10441 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
    10501 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
    10561 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
    10621 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
    10681 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
    10741 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
    10801 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
    10861 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
    10921 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
    10981 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
    11041 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
    11101 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
    11161 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
    11221 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
    11281 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
    11341 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
    11401 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
    11461 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
    11521 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
    11581 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
    11641 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
    11701 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
    11761 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
    11821 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
    11881 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
    11941 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
    12001 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
    12061 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
    12121 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
    12181 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
    12241 aaatggacga acggataaac ct
//
